Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.

ABSTRACT: Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology. Volume or three-dimensional (3D) correlative light and electron microscopy (volume-CLEM) holds unique potential to explore cellular physiology at high-resolution ultrastructural detail across cell volumes. However, the application of volume-CLEM is hampered by limitations in throughput and 3D correlation efficiency. In order to address these limitations, we describe a novel pipeline for volume-CLEM that provides high-precision (<100 nm) registration between 3D fluorescence microscopy (FM) and 3D electron microscopy (EM) datasets with significantly increased thr

SUBMITTER: Loginov SV 

PROVIDER: S-EPMC9035751 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature