Effects of Monovalent Salt on Protein-Protein Interactions of Dilute and Concentrated Monoclonal Antibody Formulations.

ABSTRACT: In this study, we used sodium chloride (NaCl) to extensively modulate non-specific protein-protein interactions (PPI) of a humanized anti-streptavidin monoclonal antibody class 2 molecule (ASA-IgG2). The changes in PPI with varying NaCl (C_NaCl) and monoclonal antibody (mAb) concentration (C_mAb) were assessed using the diffusion interaction parameter k_D and second virial coefficient B₂₂ measured from solutions with low to moderate C_mAb. The effective structure factor S(q)_eff measured from concentrated mAb solutions using small-angle X-ray and neutron scattering (SAXS/SANS) was also used to characterize the PPI. Our results found that the nature of net PPI changed not only with C

SUBMITTER: Xu AY 

PROVIDER: S-EPMC9036246 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature