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Absolute content determination by quantitative NMR (qNMR) spectroscopy: a curious case of aldosterone.

ABSTRACT: Quantitative NMR spectroscopy has been utilized to calculate the absolute content (g g^-1) of aldosterone, which is necessary for electrolyte balance and blood pressure regulation, in commercially available materials. Explanations have been provided for many signals observed in the ¹HNMR spectrum, false interpretation of which can have significant effects if such a value is utilized for the primary calibrators in ID-LC-MS/MS ('gold standard') reference methods in clinical chemistry.

SUBMITTER: Singh N

PROVIDER: S-EPMC9036601 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Absolute content determination by quantitative NMR (qNMR) spectroscopy: a curious case of aldosterone.

Singh Neeraj N Taibon Judith J Pongratz Stephan S Geletneky Christian C

RSC advances 20210705 38

Quantitative NMR spectroscopy has been utilized to calculate the absolute content (g g<sup>-1</sup>) of aldosterone, which is necessary for electrolyte balance and blood pressure regulation, in commercially available materials. Explanations have been provided for many signals observed in the <sup>1</sup>HNMR spectrum, false interpretation of which can have significant effects if such a value is utilized for the primary calibrators in ID-LC-MS/MS ('gold standard') reference methods in clinical ch ...[more]

PMID: 35479823

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