Construction of a lysosome-targetable ratiometric fluorescent probe for H₂O₂ tracing and imaging in living cells and an inflamed model.

ABSTRACT: Hydrogen peroxide (H₂O₂), an important reactive oxygen species (ROS) with unique destructive oxidation properties, can be produced in lysosomes to fight off pathogens. Although many fluorescent probes have been developed for the detection and imaging of H₂O₂, the development of a ratiometric fluorescent probe for H₂O₂ detection and imaging in lysosomes and an inflammation model remains rather scarce. Therefore, it is important to develop an efficient tool for monitoring H₂O₂ in inflamed tissues to evaluate the physiological and pathological relationship between inflammation and lysosomal H₂O₂. In this work, a new naphthalimide-based lysosome-targeting fluorescent probe (NPT-H₂

SUBMITTER: Zhou R 

PROVIDER: S-EPMC9036682 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature