Dataset Information

Protection of lead-induced cytotoxicity using paramagnetic nickel-insulin quantum clusters.

ABSTRACT: Pb-toxicity is associated with inflammation which leads to delay in wound healing. Pb²⁺ utilizes calcium ion channels to enter the cell. Therefore, to achieve effective healing in a Pb-poisoned system, capturing Pb²⁺ from the circulatory system would be an effective approach without hampering the activity of the calcium ion channel. In this work insulin-nickel fluorescent quantum clusters (INiQCs) have been synthesized and used for the specific detection of Pb²⁺ ions in vitro and in cell-free systems. INiQCs (0.09 μM) can detect Pb²⁺ concentrations as low as 10 pM effectively in a cell-free system using the fluorescence turn-off method. In vitro INiQCs (0.45 μM) can detect Pb²⁺ concentrations as low as 1 μM. INiQCs also promote wound healing which can easily be monitored using the bright fluorescence of INiQCs. INiQCs also help to overcome the wound recovery inhibitory effect of Pb²⁺ in vitro using lead nitrate. This work helps to generate effective biocompatible therapeutics for wound recovery in Pb²⁺ poisoned individuals.

SUBMITTER: Sharda D

PROVIDER: S-EPMC9036906 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Protection of lead-induced cytotoxicity using paramagnetic nickel-insulin quantum clusters.

Sharda Deepinder D Attri Komal K Kaur Pawandeep P Choudhury Diptiman D

RSC advances 20210714 40

Pb-toxicity is associated with inflammation which leads to delay in wound healing. Pb2+ utilizes calcium ion channels to enter the cell. Therefore, to achieve effective healing in a Pb-poisoned system, capturing Pb2+ from the circulatory system would be an effective approach without hampering the activity of the calcium ion channel. In this work insulin-nickel fluorescent quantum clusters (INiQCs) have been synthesized and used for the specific detection of Pb2+ ...[more]

PMID: 35481039

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Project description:Naturally occurring paramagnetic species (PS), such as free radicals and paramagnetic metalloproteins, play an essential role in a multitude of critical physiological processes including metabolism, cell signaling, and immune response. These highly dynamic species can also act as intrinsic biomarkers for a variety of disease states, while synthetic paramagnetic probes targeted to specific sites on biomolecules enable the study of functional information such as tissue oxygenation and redox status in living systems. The work presented herein describes a new sensing method that exploits the spin-dependent emission of photoluminescence (PL) from an ensemble of nitrogen-vacancy centers in diamond for rapid, nondestructive detection of PS in living systems. Uniquely this approach involves simple measurement protocols that assess PL contrast with and without the application of microwaves. The method is demonstrated to detect concentrations of paramagnetic salts in solution and the widely used magnetic resonance imaging contrast agent gadobutrol with a limit of detection of less than 10 attomol over a 100 μm × 100 μm field of view. Real-time monitoring of changes in the concentration of paramagnetic salts is demonstrated with image exposure times of 20 ms. Further, dynamic tracking of chemical reactions is demonstrated via the conversion of low-spin cyanide-coordinated Fe3+ to hexaaqua Fe3+ under acidic conditions. Finally, the capability to map paramagnetic species in model cells with subcellular resolution is demonstrated using lipid membranes containing gadolinium-labeled phospholipids under ambient conditions in the order of minutes. Overall, this work introduces a new sensing approach for the realization of fast, sensitive imaging of PS in a widefield format that is readily deployable in biomedical settings. Ultimately, this new approach to nitrogen vacancy-based quantum sensing paves the way toward minimally invasive real-time mapping and observation of free radicals in in vitro cellular environments.

Dataset Information

Protection of lead-induced cytotoxicity using paramagnetic nickel-insulin quantum clusters.

Publications

Protection of lead-induced cytotoxicity using paramagnetic nickel-insulin quantum clusters.

Similar Datasets

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