The eIg technology to generate Ig-like bispecific antibodies.

ABSTRACT: Bispecific antibodies have emerged as therapeutic molecules with a multitude of modes of action and applications. Here, we present a novel approach to solve the light-chain problem for the generation of bispecific Ig-like antibodies using the second constant domain of IgE (EHD2) genetically modified to force heterodimerization. This was achieved by introducing a C14S mutation in one domain and a C102S mutation in the other domain, which removed of one of the crossover disulfide bonds. Substituting the C_H1 and C_L domains of an antigen binding fragment (Fab) with these heterodimerizing EHD2 (hetEHD2) domains resulted in Fab-like building blocks (eFab). These eFabs were used to generate different bispecific antibodies of varying valency and molecular composition employin

SUBMITTER: Kuhl L 

PROVIDER: S-EPMC9037507 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature