A new G-triplex-based strategy for sensitivity enhancement of the detection of endonuclease activity and inhibition.

ABSTRACT: EcoRI is an important biomacromolecule in live cells and protects bacterial cells against foreign DNA. In this work, we developed a simple and convenient G-triplex (G3) (5'-TGGGAAGGGAGGGAATTCCCT-3')-based colorimetric assay for the rapid and selective detection of EcoRI activity and inhibition. The sequence specifically responds to EcoRI in the presence of K⁺ and hemin to form a G-triplex/hemin complex. Taking advantage of G-triplex, EcoRI activity was investigated under the optimized conditions. The absorption intensity ratio displayed a linear relationship against the concentration of EcoRI in the range 0 to 100 U mL^-1, and the detection limit was 5.7 U mL^-1. Furthermore, G3 showed good selectivity, and the ability to be used to screen for EcoRI inhibitor

SUBMITTER: Gao C 

PROVIDER: S-EPMC9037997 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature