Non-invasive Raman spectroscopy for time-resolved in-line lipidomics.

ABSTRACT: Oil-producing yeast cells are a valuable alternative source for palm oil production and, hence, may be one important piece of the puzzle for a more sustainable future. To achieve a high-quality product, the lipid composition inside oil-producing yeast cells is a crucial parameter for effective process control. Typically, the lipid composition is determined by off-line gas chromatography. A faster, less cumbersome approach is proposed here, by using non-invasive in-line Raman spectroscopy. A fed-batch fermentation of C. oleaginosus - a well-known oleaginous yeast - is used as model experiment to highlight the potential of Raman spectroscopy for in-line lipidomics. The temporal progression of biomass formation, lipid production and glucose consumption are determined based on PLS-regre

SUBMITTER: Wieland K 

PROVIDER: S-EPMC9038134 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature