Replication kinetics and infectivity of SARS-CoV-2 variants of concern in common cell culture models.

ABSTRACT:

Background

During the ongoing Covid-19 pandemic caused by the emerging virus SARS-CoV-2, research in the field of coronaviruses has expanded tremendously. The genome of SARS-CoV-2 has rapidly acquired numerous mutations, giving rise to several Variants of Concern (VOCs) with altered epidemiological, immunological, and pathogenic properties.

Methods

As cell culture models are important tools to study viruses, we investigated replication kinetics and infectivity of SARS-CoV-2 in the African Green Monkey-derived Vero E6 kidney cell line and the two human cell lines Caco-2, a colon epithelial carcinoma cell line, and the airway epithelial carcinoma cell line Calu-3. We assessed viral RNA copy numbers and infectivity of viral particles in cell culture supernatants at different ti

SUBMITTER: Mautner L 

PROVIDER: S-EPMC9038516 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature