Novel role of UHRF1 in the epigenetic repression of the latent HIV-1.

ABSTRACT:

Background

The multiplicity, heterogeneity, and dynamic nature of human immunodeficiency virus type-1 (HIV-1) latency mechanisms are reflected in the current lack of functional cure for HIV-1. Accordingly, all classes of latency-reversing agents (LRAs) have been reported to present variable ex vivo potencies. Here, we investigated the molecular mechanisms underlying the potency variability of one LRA: the DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-AzadC).

Methods

We employed epigenetic interrogation methods (electrophoretic mobility shift assays, chromatin immunoprecipitation, Infinium array) in complementary HIV-1 infection models (latently-infected T-cell line models, primary CD4⁺ T-cell models and ex vivo cultures of PBMCs from HIV⁺ individu

SUBMITTER: Verdikt R 

PROVIDER: S-EPMC9038550 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature