Transient Cell Cycle Induction in Cardiomyocytes to Treat Subacute Ischemic Heart Failure.

ABSTRACT:

Background

The regenerative capacity of the heart after myocardial infarction is limited. Our previous study showed that ectopic introduction of 4 cell cycle factors (4F; CDK1 [cyclin-dependent kinase 1], CDK4 [cyclin-dependent kinase 4], CCNB [cyclin B1], and CCND [cyclin D1]) promotes cardiomyocyte proliferation in 15% to 20% of infected cardiomyocytes in vitro and in vivo and improves cardiac function after myocardial infarction in mice.

Methods

Using temporal single-cell RNA sequencing, we aimed to identify the necessary reprogramming stages during the forced cardiomyocyte proliferation with 4F on a single cell basis. Using rat and pig models of ischemic heart failure, we aimed to start the first preclinical testing to introduce 4F gene therapy as a candidate for the tre

SUBMITTER: Abouleisa RRE 

PROVIDER: S-EPMC9038650 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature