PRKDC promotes hepatitis B virus transcription through enhancing the binding of RNA Pol II to cccDNA.

ABSTRACT: Hepatitis B virus infection remains a major health problem worldwide due to its high risk of liver failure and hepatocellular carcinoma. Covalently closed circular DNA (cccDNA), which is present as an individual minichromosome, serves as the template for transcription of all viral RNAs and pla ays critical role in viral persistence. Therefore, there is an urgent need to gain broader insight into the transcription regulation of cccDNA. Here, we combined a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with an engineered ascorbate peroxidase 2 (APEX2) to identify cccDNA associated proteins systematically in living cells. By functional screening, we verified that protein kinase, DNA-activated, catalytic subunit (PRKDC) was an effective activator of HBV cccDNA tran

SUBMITTER: Fan Y 

PROVIDER: S-EPMC9038722 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature