Shotgun metagenomic sequencing revealed the prebiotic potential of a grain-based diet in mice.

ABSTRACT: In the present study, we elucidated the effect of grain-based (GB) diet containing both soluble and insoluble fibers and purified ingredients-based (PIB) diet containing only insoluble fiber, namely cellulose on mice gut microbiome using whole shotgun based metagenomic sequencing. Although the fiber content in both diet types is the same (5%) the presence of soluble fiber only in the GB diet differentiates it from the PIB diet. The taxonomic analysis of sequenced reads reveals a significantly higher enrichment of probiotic Lactobacilli in the GB group as compared to the PIB group. Further, the enhancement of energy expensive cellular processes namely, cell cycle control, cell division, chromosome partitioning, and transcription is observed in the GB group which could be due to the metaboli

SUBMITTER: Jangid A 

PROVIDER: S-EPMC9038746 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature