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Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells.

ABSTRACT: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).

SUBMITTER: Yang J

PROVIDER: S-EPMC9038768 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells.

Yang Jiwen J Hu Minjie M Wang Yongyu Y

STAR protocols 20220413 2

In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of <i>FLI1</i> and/or <i>YAP</i> in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution o ...[more]

PMID: 35496784

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