Whole genome sequencing and phylogenomic analyses of a novel glufosinate-tolerant Pseudomonas species.

ABSTRACT: A novel glufosinate-tolerant Pseudomonas sp. LA21, was isolated from soil samples of an oil palm plantation with a long history of glufosinate application. The genome of Pseudomonas sp. LA21 was sequenced with 150 bp paired-end conducted using Illumina sequencing technology. De novo genome assembly was performed using SPAdes, ABySS, and Velvet assemblers. Phylogenetic analysis using 16S rRNA gene sequence showed that Pseudomonas sp. LA21 was closely related to Pseudomonas nitroreducens ATCC 33634. Multilocus sequence analysis (MLSA) based on four bacterial housekeeping genes (16S rRNA, gyr B, rpo B, and rpo D) was conducted together with 138 reference genomes of Pseudomonas species. The phylogenetic tree derived f

SUBMITTER: Wee WY 

PROVIDER: S-EPMC9038976 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature