Dissecting the dominant hot spring microbial populations based on community-wide sampling at single-cell genomic resolution.

ABSTRACT: With advances in DNA sequencing and miniaturized molecular biology workflows, rapid and affordable sequencing of single-cell genomes has become a reality. Compared to 16S rRNA gene surveys and shotgun metagenomics, large-scale application of single-cell genomics to whole microbial communities provides an integrated snapshot of community composition and function, directly links mobile elements to their hosts, and enables analysis of population heterogeneity of the dominant community members. To that end, we sequenced nearly 500 single-cell genomes from a low diversity hot spring sediment sample from Dewar Creek, British Columbia, and compared this approach to 16S rRNA gene amplicon and shotgun metagenomics applied to the same sample. We found that the broad taxonomic profiles were similar a

SUBMITTER: Bowers RM 

PROVIDER: S-EPMC9039060 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature