The macroscopic limit to synchronization of cellular clocks in single cells of Neurospora crassa.

ABSTRACT: We determined the macroscopic limit for phase synchronization of cellular clocks in an artificial tissue created by a "big chamber" microfluidic device to be about 150,000 cells or less. The dimensions of the microfluidic chamber allowed us to calculate an upper limit on the radius of a hypothesized quorum sensing signal molecule of 13.05 nm using a diffusion approximation for signal travel within the device. The use of a second microwell microfluidic device allowed the refinement of the macroscopic limit to a cell density of 2166 cells per fixed area of the device for phase synchronization. The measurement of averages over single cell trajectories in the microwell device supported a deterministic quorum sensing model identified by ensemble methods for clock phase synchronization. A strong

SUBMITTER: Cheong JH 

PROVIDER: S-EPMC9039089 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature