Dataset Information

Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant.

ABSTRACT:

Background and aim

Plantago major has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of P. major are limited.

Experimental procedure

The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of P. major (IPM) were evaluated using Complete Freund's Adjuvant (CFA)-induced arthritis induced in female Wistar rat by CFA. Diclofenac was used as a positive control. The volume of paw oedema, white blood cell count, lymphocytes, neutrophils, expression of TNF-α and Interleukin-6 and the histopathological features of the joint tissues were assessed to characterise IPM activity.

Results

The IPM extract at doses of 280 and 420 mg/kg BW and diclofenac inhibited paw oedema by 15.70 %, 15.94 % and 19.71 % respectively. IPM also reduced the incidence of arthritis and arthritic index. Unlike untreated rats, animals treated with IPM showed a significant decrease in the number of neutrophils and decreased expression of TNF-α and Interleukin-6. Histopathological examination showed a reduction in the number of inflammatory cells and hyperplasia of the synovium after IPM treatment.

Conclusion

This study showed that P. major displays anti-rheumatoid arthritis activity.

SUBMITTER: Triastuti A

PROVIDER: S-EPMC9039107 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Anti-rheumatoid activity of a hexane-insoluble fraction from <i>Plantago major</i> in female Wistar rats induced by Complete Freund's Adjuvant.

Triastuti Asih A Pradana Dimas Adhi DA Saputra Didit Erwin DE Lianika Namira N Wicaksono Hilmy Ramadhan HR Anisari Thessy Dewi TD Widyarini Sitarina S

Journal of traditional and complementary medicine 20210728 3

<h4>Background and aim</h4><i>Plantago major</i> has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of <i>P. major</i> are limited.<h4>Experimental procedure</h4>The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of <i>P. major</i> (IPM) were evaluated using Complete Freund's Adjuvant (CFA)-induced arthritis induced in female Wistar rat by CFA. Diclofenac was used as a positive control. The volume of pa ...[more]

PMID: 35493315

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