Dicalcium silicate-induced mitochondrial dysfunction and autophagy-mediated macrophagic inflammation promotes osteogenic differentiation of BMSCs.

ABSTRACT: Dicalcium silicate (Ca₂SiO₄, C₂S) has osteogenic potential but induces macrophagic inflammation. Mitochondrial function plays a vital role in macrophage polarization and macrophagic inflammation. The mitochondrial function of C₂S-treated macrophages is still unclear. This study hypothesized: (i) the C₂S modulates mitochondrial function and autophagy in macrophages to regulate macrophagic inflammation, and (ii) C₂S-induced macrophagic inflammation regulates osteogenesis. We used RAW264.7 cells as a model of macrophage. The C₂S (75-150 μg/ml) extract was used to analyze the macrophagic mitochondrial function and macrophage-mediated effect on osteogenic differentiation of mouse bone marrow-derived mesenchymal stem ce

SUBMITTER: Luo Q 

PROVIDER: S-EPMC9039510 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature