Mycobiome detection from a single subterranean gametophyte using metabarcoding techniques.

ABSTRACT:

Premise

Several ferns and lycophytes produce subterranean gametophytes, including the Ophioglossaceae, Psilotaceae, and some members of the Schizaeaceae, Gleicheniaceae, and Lycopodiaceae. Despite the surge in plant-microbiome research, which has been particularly boosted by high-throughput sequencing techniques, the microbiomes of these inconspicuous fern gametophytes have rarely been examined. The subterranean gametophytes are peculiar due to their achlorophyllous nature, which makes them rely on fungi to obtain nutrients. Furthermore, the factors that shape the fungal communities (mycobiomes) of fern gametophytes have not been examined in depth.

Methods and results

We present a workflow to study the mycobiome of the achlorophyllous gametophytes of Ophioderma pendulum</

SUBMITTER: Chen KH 

PROVIDER: S-EPMC9039796 | biostudies-literature | 2022 Mar-Apr

REPOSITORIES: biostudies-literature