Prime-boost with Chikungunya virus E2 envelope protein combined with Poly (I:C) induces specific humoral and cellular immune responses.

ABSTRACT: Chikungunya virus (CHIKV) is an arbovirus transmitted to humans mainly by the bite of infected Aedes aegypti and Aedes albopictus mosquitoes. CHIKV illness is characterized by fever and long-lasting arthritic symptoms, and in some cases it is a deadly disease. The CHIKV envelope E2 (E2_CHIKV) glycoprotein is crucial for virus attachment to the cell. Furthermore, E2_CHIKV is the immunodominant protein and the main target of neutralizing antibodies. To date, there is no available prophylactic vaccine or specific treatment against CHIKV infection. Here, we designed and produced a DNA vaccine and a recombinant protein containing a consensus sequence of E2_CHIKV. C57BL/6 mice immunized twice with the E2_CHIKV recombinant protein in the prese

SUBMITTER: Amaral MP 

PROVIDER: S-EPMC9040086 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature