Characterization of Endogenous and Extruded H₂S and Small Oxoacids of Sulfur (SOS) in Cell Cultures.

ABSTRACT: This report characterizes and quantifies endogenous hydrogen sulfide (H₂S) and small oxoacids of sulfur (SOS = HOSH, HOSOH) in a panel of cell lines including human cancer (A375 melanoma cells, HeLa cervical cells) and noncancer (HEK293 embryonic kidney cells), as well as E. coli DH5α and S. cerevisiae S288C. The methodology used is a translation of well-studied nucleophilic and electrophilic traps for cysteine and oxidized cysteines residues to target small molecular weight sulfur species; mass spectrometric analysis allows for species quantification. The observed intracellular concentrations of H₂S and SOS vary in different cell types, from nanomolar to femtomolar, typically with H₂S > HOSOH > HOSH. We propose the term sulfome, a subset of t

SUBMITTER: Scrivner O 

PROVIDER: S-EPMC9040188 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature