Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity.

ABSTRACT:

Background

The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system. Features such as recognition of T-rich protospacer-adjacent motif (PAM) and generation of sticky breaks, as well as amenability for multiplex editing in a single crRNA and lower off-target nuclease activity, broaden the targeting scope of available tools and enable more accurate genome editing. However, the widespread use of the nuclease for gene editing, especially in clinical applications, is hindered by insufficient activity and specificity despite previous efforts to improve the system. Currently reported Cas12a variants achieve high activity with a compromise of specificity. Here, we used structure-guided protein engineering to improv

SUBMITTER: Huang H 

PROVIDER: S-EPMC9040236 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature