A novel peptide inhibitor of Dll4-Notch1 signalling and its pro-angiogenic functions.

ABSTRACT:

Background and purpose

The Dll4-Notch1 signalling pathway plays an important role in sprouting angiogenesis, vascular remodelling and arterial or venous specificity. Genetic or pharmacological inhibition of Dll4-Notch1 signalling leads to excessive sprouting angiogenesis. However, transcriptional inhibitors of Dll4-Notch1 signalling have not been described.

Experimental approach

We designed a new peptide targeting Notch signalling, referred to as TAT-ANK, and assessed its effects on angiogenesis. In vitro, tube formation and fibrin gel bead assay were carried out, using human umbilical vein endothelial cells (HUVECs). In vivo, Matrigel plug angiogenesis assay, a developmental retinal model and tumour models in mice were used. The mechanisms underlying TAT-ANK activity were i

SUBMITTER: Zhu G 

PROVIDER: S-EPMC9040338 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature