Deciphering the interaction of flavones with calf thymus DNA and octamer DNA sequence (CCAATTGG)₂.

ABSTRACT: We investigated the interaction of three flavone compounds, baicalein, chrysin and flavone with calf thymus DNA and octamer DNA sequence (CCAATTGG)₂. The binding mechanisms of the flavone compounds with both DNA were unveiled using biophysical, thermodynamic and molecular modelling techniques. Absorption and fluorescence titrations confirm the formation of the DNA complexes along with the extent of interaction. Absorption data proposed an intercalation mode of binding. Fluorescence displacement assays using ethidium bromide and Hoechst 33258 data supports a partial intercalation. Potassium iodide quenching substantiated this finding. Circular dichroism data revealed major structural changes on binding with flavones which can arise from intercalation partially or in a tilted arra

SUBMITTER: Kumar S 

PROVIDER: S-EPMC9040621 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature