High-Content Screening and Computational Prediction Reveal Viral Genes That Suppress the Innate Immune Response.

ABSTRACT: Suppression of the host innate immune response is a critical aspect of viral replication. Upon infection, viruses may introduce one or more proteins that inhibit key immune pathways, such as the type I interferon pathway. However, the ability to predict and evaluate viral protein bioactivity on targeted pathways remains challenging and is typically done on a single-virus or -gene basis. Here, we present a medium-throughput high-content cell-based assay to reveal the immunosuppressive effects of viral proteins. To test the predictive power of our approach, we developed a library of 800 genes encoding known, predicted, and uncharacterized human virus genes. We found that previously known immune suppressors from numerous viral families such as Picornaviridae and Flaviviridae rec

SUBMITTER: Ng TL 

PROVIDER: S-EPMC9040872 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature