Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst.

ABSTRACT: Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity.

SUBMITTER: Zhang QH 

PROVIDER: S-EPMC9041136 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature