Isolation and Characterization of vB_PagP-SK1, a T7-Like Phage Infecting Pantoea agglomerans.

ABSTRACT: Background: Pantoea is a genus within the Enterobacterales whose members encompass free-living and host-associated lifestyles. Despite our growing understanding of the role of mobile genetic elements in the biology, ecology, and evolution of this bacterial group, few Pantoea bacteriophages have been identified and characterized. Materials and Methods: A bacteriophage that could infect Pantoea agglomerans was isolated from barnyard soil. We used electron microscopy and complete genome sequencing to identify the viral family, and evaluated its host range across 10 different Pantoea species groups using both bacterial lawn and phage lawn assays. The latter assays were carried out using a scalable microplate assay to increase throu

SUBMITTER: McDougall DL 

PROVIDER: S-EPMC9041449 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature