Isolation and Characterization of vB_MsmS_Celfi: A New <i>Mycobacterium tuberculosis</i> Bacteriophage.

ABSTRACT: Introduction: Because of the clinical relevance of Mycobacteria, and from a therapeutic perspective, there is an increasing interest to study phages that infect bacteria belonging to this genus. Materials and Methods: A phage was isolated from a soil sample, using Mycobacterium smegmatis as host. Its characterization included sequencing, annotation, and analysis of the genome, host range determination, and electron microscopy imaging. Results: Mycobacterium phage vB_MsmS_Celfi is a temperate phage able to infect Mycobacterium tuberculosis with high efficiency. From electron microscopy images, Celfi belongs to the Siphoviridae family. Genome analysis classified phage Celfi into cluster L, subcluster L2 of Actinobac

SUBMITTER: Payaslian F 

PROVIDER: S-EPMC9041486 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature