Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.

ABSTRACT: Cortico-striatal projections are critical components of forebrain circuitry that regulate motivated behaviors. To enable the study of the human cortico-striatal pathway and how its dysfunction leads to neuropsychiatric disease, we developed a method to convert human pluripotent stem cells into region-specific brain organoids that resemble the developing human striatum and include electrically active medium spiny neurons. We then assembled these organoids with cerebral cortical organoids in three-dimensional cultures to form cortico-striatal assembloids. Using viral tracing and functional assays in intact or sliced assembloids, we show that cortical neurons send axonal projections into striatal organoids and form synaptic connections. Medium spiny neurons mature electrophysiologically follo

SUBMITTER: Miura Y 

PROVIDER: S-EPMC9042317 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature