Customized peptidoglycan surfaces to investigate innate immune recognition via surface plasmon resonance.

ABSTRACT: Glycan-protein interactions facilitate some of the most important biomolecular processes in and between cells. They are involved in different cellular pathways, cell-cell interactions and associated with many diseases, making these interactions of great interest. However, their structural and functional diversity poses great challenges in studying them at the molecular level. Surface plasmon resonance (SPR) technology presents great advantages to study glycan-protein interactions due to its superior sensitivity, ability to monitor real-time interactions, relatively simple data interpretation, and most importantly, direct measurement of binding without a need for fluorescent labeling. Here, another dimensionality of SPR in studying glycan-protein interactions is demonstrated via examples of

SUBMITTER: Le HT 

PROVIDER: S-EPMC9042648 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature