Rational design of a selective and sensitive "turn-on" fluorescent probe for monitoring and imaging hydrogen peroxide in living cells.

ABSTRACT: As one type of reactive oxygen species (ROS), hydrogen peroxide (H₂O₂) plays a key role in regulating a variety of cellular functions. Herein, a fluorescent probe N-Py-BO was well designed and synthesized and its ability for detecting H₂O₂ by fluorescence intensity was evaluated. In the design, the arylboronate ester group was acted as a reaction site for H₂O₂. Upon reaction with H₂O₂ under physiological conditions, the boronate moiety in the probe was oxidized, followed by detachment from the probe and as a result, a "turn-on" fluorescence response for H₂O₂ was acquired. Due to the D-A structure formation between N,N'-dimethylaminobenzene and the -CN group and the li

SUBMITTER: Lu J 

PROVIDER: S-EPMC9042858 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature