Ratiometric fluorescence assay based on carbon dots and Cu²⁺-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox.

ABSTRACT: The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as a donor are rarely reported as a drug monitor. In this work, CDs with an appropriate emitting wavelength at 480 nm and excitation around 370 nm were prepared by hydrothermal approach and HCl post-treatment. O-Phenylenediamine (OPD) can be oxidized by Cu²⁺ to produce yellow fluorescent 2,3-diaminophenazine (oxOPD) in the system of Cu²⁺ and OPD (Cu-OPD). Correspondingly, a remarkable FRET from CDs to oxOPD in the system of CDs, Cu²⁺ and OPD (CDs-Cu-OPD) was fabrica

SUBMITTER: Miao CF 

PROVIDER: S-EPMC9042915 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature