Elastase- and LPS-Exposed Cpa3^Cre/+ and ST2^-/- Mice Develop Unimpaired Obstructive Pulmonary Disease.

ABSTRACT: IL-33 and its receptor ST2, as well as mast cells and their mediators, have been implicated in the development of chronic obstructive pulmonary disease (COPD). However, whether mast cells and the ST2 receptor play a critical role in COPD pathophysiology remains unclear. Here, we performed repeated intranasal administrations of porcine pancreatic elastase and LPS for four weeks to study COPD-like disease in wildtype, ST2-deficient, and Cpa3^Cre/+ mice, which lack mast cells and have a partial reduction in basophils. Alveolar enlargement and changes in spirometry-like parameters, e.g. increased dynamic compliance and decreased expiratory capacity, were evident one day after the final LPS challenge and worsened over time. The elastase/LPS model also induced mild COPD-like airway inf

SUBMITTER: Cardenas EI 

PROVIDER: S-EPMC9043106 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature