Molecular mechanism of m⁶A methylation of circDLC1 mediated by RNA methyltransferase METTL3 in the malignant proliferation of glioma cells.

ABSTRACT: Glioma is an intracranial malignant tumor and remains largely incurable. Circular RNAs are prominent modulators in glioma progression. This study investigated the function of circular RNA DLC1 (circDLC1) in the malignant proliferation of glioma cells. circDLC1 expression in glioma tissues and cells was determined using RT-qPCR. The effect of circDLC1 on the malignant proliferation of glioma cells was analyzed using CCK-8, colony formation, and EdU staining assays. METTL3, miR-671-5p, and CTNNBIP1 expressions were determined. N⁶ methyladenosine (m⁶A) level of circDLC1 was analyzed using MeRIP. The binding relationship between miR-671-5p and circDLC1 or CTNNBIP1 was verified using RNA pull-down and dual-luciferase assays. A xenograft tumor model was established in nude

SUBMITTER: Wu Q 

PROVIDER: S-EPMC9043209 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature