Self-adjuvanting nanovaccines boost lung-resident CD4⁺ T cell immune responses in BCG-primed mice.

ABSTRACT: Heterologous vaccine regimens could extend waning protection in the global population immunized with Mycobacterium bovis Bacille Calmette-Guerin (BCG). We demonstrate that pulmonary delivery of peptide nanofibers (PNFs) bearing an Ag85B CD4⁺ T cell epitope increased the frequency of antigen-specific T cells in BCG-primed mice, including heterogenous populations with tissue resident memory (Trm) and effector memory (Tem) phenotype, and functional cytokine recall. Adoptive transfer of dendritic cells pulsed with Ag85B-bearing PNFs further expanded the frequency and functional repertoire of memory CD4⁺ T cells. Transcriptomic analysis suggested that the adjuvanticity of peptide nanofibers is, in part, due to the release of damage-associated molecular patterns. A single b

SUBMITTER: Files MA 

PROVIDER: S-EPMC9043212 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature