Regulation of Liver Regeneration by Hepatocyte O-GlcNAcylation in Mice.

ABSTRACT:

Background & aims

The liver has a unique capacity to regenerate after injury in a highly orchestrated and regulated manner. Here, we report that O-GlcNAcylation, an intracellular post-translational modification regulated by 2 enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), is a critical termination signal for liver regeneration following partial hepatectomy (PHX).

Methods

We studied liver regeneration after PHX on hepatocyte specific OGT and OGA knockout mice (OGT-KO and OGA-KO), which caused a significant decrease (OGT-KO) and increase (OGA-KO) in hepatic O-GlcNAcylation, respectively.

Results

OGA-KO mice had normal regeneration, but the OGT-KO mice exhibited substantial defects in termination of liver regeneration with increased liver injury, sustained ce

SUBMITTER: Robarts DR 

PROVIDER: S-EPMC9043307 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature