Controlling the adsorption of osteopontin for mediating cell behaviour by using self-assembled monolayers with varying surface chemistry.

ABSTRACT: Osteopontin (OPN) is an important protein for mediating cell behaviour on biomaterials. However, the interactions between the chemical groups on the biomaterial surface and OPN still need to be further clarified, which has restricted the application of OPN in biomaterial functionalization. In the present study, we developed different self-assembled monolayers (SAMs) with specific chemical groups, including SAMs-OH, SAMs-OEG, SAMs-COOH, SAMs-NH₂, and SAMs-PO₃H₂, to study the behavior of OPN on these SAMs. The results showed that SAMs-NH₂ could strongly adsorb OPN, and the amount of protein was highest on this material. Meanwhile, the lowest amount of OPN was present on SAMs-OEG. Interestingly, the unit-mass trend of bound OPN monoclonal antibodies

SUBMITTER: Chen Z 

PROVIDER: S-EPMC9043331 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature