A sandwich SERS immunoassay platform based on a single-layer Au-Ag nanobox array substrate for simultaneous detection of SCCA and survivin in serum of patients with cervical lesions.

ABSTRACT: The evaluation of tumor biomarkers in blood specimens is vital for patients with cervical lesions. Herein, an ultrasensitive surface enhanced Raman scattering (SERS) platform was proposed for simultaneous detection of cervical-lesion-related serum biomarkers. Raman reporter labeled Au-Ag nanoshells (Au-AgNSs) acted as SERS tags and an Au-Ag nanobox (Au-AgNB) array substrate prepared by the oil-water interface self-assembly method was used as a capture substrate. This single-layer Au-AgNB array substrate was proved to have exceptional uniformity by atomic force microscopy and SERS mapping. Numerous "hot spots" and specific adsorption surfaces offered by the Au-AgNB array substrate were confirmed by the finite difference time domain method, which could generate a SERS signal in electromagnet

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9043334 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature