Validation of a multilocus genotyping scheme for subtyping Cryptosporidium parvum for epidemiological purposes.

ABSTRACT: Subtyping Cryptosporidium parvum for outbreak investigations or epidemiological surveillance usually relies on DNA sequence analysis of a gene coding for a 60 KDa glycoprotein (gp60). Although gp60 can be useful for allelic discrimination and to help investigate sources and routes of transmission, the presence of common subtypes and recombination during the parasite's sexual life-cycle demand a multilocus-based method for more discriminatory genotyping. While whole genome sequencing would provide the ultimate approach, it is a time consuming and expensive option for faecal parasites such as Cryptosporidium that occur at low density and are difficult to propagate routinely. In this study, we selected and evaluated a panel of previously identified variable-number

SUBMITTER: Robinson G 

PROVIDER: S-EPMC9043402 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature