Improving the catalytic performance of Pichia pastoris whole-cell biocatalysts by fermentation process.

ABSTRACT: Whole-cell biocatalysts have a wide range of applications in many fields. However, the transport of substrates is tricky when applying whole-cell biocatalysts for industrial production. In this research, P. pastoris whole-cell biocatalysts were constructed for rebaudioside A synthesis. Sucrose synthase was expressed intracellularly while UDP-glycosyltransferase was displayed on the cell wall surface simultaneously. As an alternative method, a fermentation process is applied to relieve the substrate transport-limitation of P. pastoris whole-cell biocatalysts. This fermentation process was much simpler, more energy-saving, and greener than additional operating after collecting cells to improve the catalytic ability of whole-cell biocatalysts. Compared with the general fe

SUBMITTER: Wang D 

PROVIDER: S-EPMC9043429 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature