Transmission electron microscopic analysis of myelination in the murine central nervous system.
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ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC9043565 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Transmission electron microscopic analysis of myelination in the murine central nervous system.
Wang Yan Y Sun Bo B Shibata Bradley B Guo Fuzheng F
STAR protocols 20220418 2
Myelin provides physical, neurotrophic, and metabolic support for axonal integrity. The thickness of CNS (central nervous system) myelin sheath is usually < one micrometer, which is under or near the detection threshold of the conventional light microscopy. Here, we present a high-resolution transmission electron microscopy-based protocol to assess myelination at the ultrastructural level. We describe sample preparation from mouse tissue, followed by electron microscopic imaging and CNS myelinat ...[more]
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