High Titers of Low Affinity Antibodies in COVID-19 Patients Are Associated With Disease Severity.

ABSTRACT:

Background

Almost 2 years from the beginning of the coronavirus disease 2019 (COVID-19) pandemic, there is still a lot unknown how the humoral response affects disease progression. In this study, we investigated humoral antibody responses against specific SARS-CoV2 proteins, their strength of binding, and their relationship with COVID severity and clinical information. Furthermore, we studied the interactions of the specific receptor-binding domain (RBD) in more depth by characterizing specific antibody response to a peptide library.

Materials and methods

We measured specific antibodies of isotypes IgM, IgG, and IgA, as well as their binding strength against the SARS-CoV2 antigens RBD, NCP, S1, and S1S2 in sera of 76 COVID-19 patients using surface plasmon resonance imaging.

SUBMITTER: Hendriks J 

PROVIDER: S-EPMC9043688 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature