CD154 Expression Indicates T Cell Activation Following Tetanus Toxoid Vaccination of Horses.

ABSTRACT: Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. In this study we used tetanus toxoid (TT) as a well-defined antigen to characterize antigen-reactive T cells. Six healthy adult horses received a routine booster against tetanus with an immune stimulating complex (ISCOM)-based vaccine and were followed for 28 days. TT-specific serum antibodies were quantified by ELISA and increased in all horses by day 7 after vaccination. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. CD154 detection in equine PBMC by an anti

SUBMITTER: Schnabel CL 

PROVIDER: S-EPMC9043809 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature