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Non-enzymatic electrochemical determination of salivary cortisol using ZnO-graphene nanocomposites.

ABSTRACT: Electrochemically deposited ZnO nanoparticles on a pencil graphite electrode (PGE) coated with graphene generate a noteworthy conductive and selective electrochemical sensing electrode for the estimation of cortisol. Electrochemical techniques such as cyclic voltammetry (CV) analysis and electrochemical impedance spectroscopic (EIS) tests were adopted to analyze and understand the nature of the modified sensor. Surface morphological analysis was done using various spectroscopic and microscopic techniques like X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Structural characterization was conducted by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The effect of scan rate, concentration, and cycle numbers was optimized and reported. Differential pulse voltammetric (DPV) analysis reveals that the linear range for the detection of cortisol is 5 × 10^-10M - 115 × 10^-10 M with a very low-level limit of detection value (0.15 nM). The demonstrated methodology has been excellently functional for the determination of salivary cortisol non-enzymatically at low-level concentration with enhanced selectivity despite the presence of interfering substances.

SUBMITTER: Rison S

PROVIDER: S-EPMC9043917 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Non-enzymatic electrochemical determination of salivary cortisol using ZnO-graphene nanocomposites.

Rison Sherin S Rajeev Rijo R Bhat Vinay S VS Mathews Agnus T AT Varghese Anitha A Hegde Gurumurthy G

RSC advances 20211124 60

Electrochemically deposited ZnO nanoparticles on a pencil graphite electrode (PGE) coated with graphene generate a noteworthy conductive and selective electrochemical sensing electrode for the estimation of cortisol. Electrochemical techniques such as cyclic voltammetry (CV) analysis and electrochemical impedance spectroscopic (EIS) tests were adopted to analyze and understand the nature of the modified sensor. Surface morphological analysis was done using various spectroscopic and microscopic t ...[more]

PMID: 35498093

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Project description:Photocatalysts suffer from a lack of separation of photogenerated excitons due to the fast recombination of charge carriers, so a strong synergistic effect exhibited by photocatalysts is promising for effective photocatalysis. Herein, we have synthesized efficient visible light functionalized graphene oxide nanosheet (FGS)/ZnO nanocomposite photocatalysts via a simple and economical approach with large scale production for practical applications. A series of nanocomposites (FGS/ZnO NCs) with different amounts by weight of graphene oxide (GO) have been synthesized via a facile solution route followed by calcination under environmental conditions. The phase, purity and morphological studies of the synthesized FGS/ZnO NCs were carried out using powder X-ray diffraction (XRD) and transmission electron microscopy (TEM). The optical properties were studied using UV-visible diffuse reflectance spectroscopy (DRS) and photoluminescence spectroscopy (PL). XRD results confirm the formation of a pure phase of ZnO in the FGS/ZnO NCs and TEM results show strongly adhered ZnO NPs on the surface of the FGS. DRS results confirm the extension of light absorption in the visible region while PL results confirm the effective separation of charge carriers in 0.09 wt% FGS/ZnO NCs. The synthesized photocatalyst efficiently degrades carcinogenic safranin-T dye under visible light illumination which is reported for the first time using FGS/ZnO nanocomposites. Photocatalytic studies confirm the higher photocatalytic activity of 0.09 wt% FGS/ZnO NCs (about 94.5%) towards the photodegradation of safranin-T dye in aqueous solution under visible light. The improved photocatalytic activity of 0.09 wt% FGS/ZnO NCs can be ascribed to the integrative synergistic effects of the enhanced adsorption capacity of safranin-T dye, effective separation of photogenerated excitons and effective interfacial hybridization of FGS and ZnO NCs. The generation of reactive oxygen species was confirmed using terephthalic acid as a probe molecule and a scavenger test was conducted in presence of histidine.

Dataset Information

Non-enzymatic electrochemical determination of salivary cortisol using ZnO-graphene nanocomposites.

Publications

Non-enzymatic electrochemical determination of salivary cortisol using ZnO-graphene nanocomposites.

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