Investigation of apoptosis based on fluorescence lifetime imaging microscopy with a mitochondria-targeted viscosity probe.

ABSTRACT: Cell apoptosis detection based on the functionality changes of cellular organelles, such as mitochondria, offers a quantitative method compared to morphology-based detection. However, the conventional detection methods for potential variation of the mitochondrial membrane based on fluorescence spectrum changes cannot offer a precise quantification of the degree of apoptosis. Here, a mitochondria-targeted two-photon viscosity probe (TPA-Mit), which sensitively responds to viscosity variations with fluorescence lifetime changes, is designed to detect the viscosity of mitochondria. Noteworthily, the proposed phasor fluorescence lifetime imaging microscopy (phasor-FLIM) allows for more precise quantification (in terms of smaller uncertainty) when estimating the degree of apoptosis with a micro

SUBMITTER: Zou G 

PROVIDER: S-EPMC9044426 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature