Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors.

ABSTRACT:

Background

Developmental pluripotency-associated 3 (Dppa3, also called Stella or PGC7) is a principal maternal protein specially expressed in pre-implantation embryos, embryonic stem cells (ES cells) and primordial germ cells (PGCs). It plays critical role in the regulating of DNA methylation in zygotes and oocytes. However, the effect of Dppa3 in ES cells on the stability of proteins is still unclear.

Methods

In this study, we first identified the potential interacting proteins with Dppa3 using immunoprecipitation-mass spectrometry (IP-MS). After GO analysis, we further constructed Dppa3-silenced ES cells and ES cell lines overexpressing with different lengths of Dppa3 to explore the mechanisms of Dppa3 on protein stability.

Results

IP-MS results showed that Dppa3 in

SUBMITTER: Zhao S 

PROVIDER: S-EPMC9044575 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature