Modulating DNA Repair Pathways to Diversify Genomic Alterations in Saccharomyces cerevisiae.

ABSTRACT: Nuclease based genome editing systems have emerged as powerful tools to drive genomic alterations and enhance genome evolution via precise engineering in the various human and microbial cells. However, error-prone DNA repair has not been well studied previously to generate diverse genomic alterations and novel phenotypes. Here, we systematically investigated the potential interplay between DNA double strand break (DSB) repair and genome editing tools, and found that modulating the DSB end resection proteins could significantly improve mutational efficiency and diversity without exogenous DNA template in yeast. Deleting SAE2, EXO1, or FUN30, or overexpressing MRE11-H125N (nuclease-dead allele of MRE11), for DSB end resection markedly increased the efficien

SUBMITTER: Wang Z 

PROVIDER: S-EPMC9045378 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature