Assembly and Functional Role of PACE Transporter PA2880 from Pseudomonas aeruginosa.

ABSTRACT: The recently identified proteobacterial antimicrobial compound efflux (PACE) transporters are multidrug transporters energized by the electrochemical gradient of protons. Here, we present the results of phylogenetic and functional studies on the PACE family transporter PA2880 from Pseudomonas aeruginosa. A phylogenetic analysis of the PACE family revealed that PA2880 and AceI from Acinetobacter baumannii are classified into evolutionarily distinct clades, although they both transport chlorhexidine. We demonstrate that PA2880 mainly exists as a dimer in solution, which is independent of pH, and its dimeric state is essential for its proper function. Electrogenicity studies revealed that the chlorhexidine/H⁺ antiport process is electrogenic. The function of several highly conserve

SUBMITTER: Zhao J 

PROVIDER: S-EPMC9045395 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature