Differential biomolecular recognition by synthetic vs. biologically-derived components in the stone-forming process using 3D microfluidics.

ABSTRACT: Calcium phosphate (CaP) biomineralization is the hallmark of extra-skeletal tissue calcification and renal calcium stones. Although such a multistep process starts with CaP crystal formation, the mechanism is still poorly understood due to the complexity of the in vivo system and the lack of a suitable approach to simulate a truly in vivo-like environment. Although endogenous proteins and lipids are engaged with CaP crystals in such a biological process of stone formation, most in vitro studies use synthetic materials that can display differential bioreactivity and molecular recognition by the cellular component. Here, we used our in vitro microfluidic (MF) tubular structure, which is the first completely cylindrical platform, with renal tubular cellular microen

SUBMITTER: Awuah Boadi E 

PROVIDER: S-EPMC9045411 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature